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krt8 cre ert2 17blpn j mice  (Jackson Laboratory)

 
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    Jackson Laboratory krt8 cre ert2 17blpn j mice
    A. Schematic of lineage-tracing strategy using <t>Krt8-Cre</t> <t>ERT2</t> ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.
    Krt8 Cre Ert2 17blpn J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt8+cre+ert2+17blpn+j+mice/cre+ert2+mice+ubc/bio_rxiv__64898__2026__04__28__721179-199-1-8
    Average 86 stars, based on 1 article reviews
    krt8 cre ert2 17blpn j mice - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Olfactomedin4 marks luminal progenitor cells that give rise to secretory cell lineage in the mouse cervix"

    Article Title: Olfactomedin4 marks luminal progenitor cells that give rise to secretory cell lineage in the mouse cervix

    Journal: bioRxiv

    doi: 10.64898/2026.04.28.721179

    A. Schematic of lineage-tracing strategy using Krt8-Cre ERT2 ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.
    Figure Legend Snippet: A. Schematic of lineage-tracing strategy using Krt8-Cre ERT2 ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.

    Techniques Used: RNA Expression, Staining, Marker

    A. Schematic of lineage tracing strategy using Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. B. RNA expression of tdTomato+ (red), Spdef (cyan) and Muc5b (green) in endocervical tissue from nonpregnant (diestrus) and pregnancy (GD 6,12,15, 18 and IL). All three markers were detected in the same section, with each signal displayed in separate panels. Images are representative of n≥ 3 biological replicates per time point and scale bars =100 µm. C. Schematic of workflow used to generate organoid cultures from tdTomato negative and tdTomato positive epithelial populations from the cervix of nonpregnant diestrus Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. D. Organoids generated from flow cytometry sorted tdTomato+ and tdTomato- epithelial populations. tdTomato+ derived organoids retained tdTomato signal, whereas tdTomato-progenitors formed organoids that did not express tdTomato. n = 3 biological replicates and scale bars =100 µm. E. Schematic of short-term and long-term lineage-tracing strategy using Olfm4 Cre ERT2 ; Rosa26 tdTomato mice (8-week-old females). Short-term tracing (top): Tamoxifen (Tmx) was administered at estrus, diestrus, or GD11, and tissues were collected 24-48 hrs later at the subsequent time points (estrus to diestrus, diestrus to estrus, GD11 to GD12). Long-term tracing (bottom): Tmx was administered at the NP stage, followed by a first pregnancy. After the first pregnancy, tissues were collected at two stages of the estrous cycle, or on GD15 of a second pregnancy. F. RNAscope images of endocervix from short-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In short-term tracing, tdTomato⁺ cells are present and co-localize with goblet markers during diestrus (first column) and on GD12 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm. G. RNAscope images of endocervix from long-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In long-term tracing, tdTomato⁺ cells persist and give rise to secretory goblet cells that express Spdef and Muc5b in diestrus (second column) and in a second pregnancy at GD15 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm.
    Figure Legend Snippet: A. Schematic of lineage tracing strategy using Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. B. RNA expression of tdTomato+ (red), Spdef (cyan) and Muc5b (green) in endocervical tissue from nonpregnant (diestrus) and pregnancy (GD 6,12,15, 18 and IL). All three markers were detected in the same section, with each signal displayed in separate panels. Images are representative of n≥ 3 biological replicates per time point and scale bars =100 µm. C. Schematic of workflow used to generate organoid cultures from tdTomato negative and tdTomato positive epithelial populations from the cervix of nonpregnant diestrus Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. D. Organoids generated from flow cytometry sorted tdTomato+ and tdTomato- epithelial populations. tdTomato+ derived organoids retained tdTomato signal, whereas tdTomato-progenitors formed organoids that did not express tdTomato. n = 3 biological replicates and scale bars =100 µm. E. Schematic of short-term and long-term lineage-tracing strategy using Olfm4 Cre ERT2 ; Rosa26 tdTomato mice (8-week-old females). Short-term tracing (top): Tamoxifen (Tmx) was administered at estrus, diestrus, or GD11, and tissues were collected 24-48 hrs later at the subsequent time points (estrus to diestrus, diestrus to estrus, GD11 to GD12). Long-term tracing (bottom): Tmx was administered at the NP stage, followed by a first pregnancy. After the first pregnancy, tissues were collected at two stages of the estrous cycle, or on GD15 of a second pregnancy. F. RNAscope images of endocervix from short-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In short-term tracing, tdTomato⁺ cells are present and co-localize with goblet markers during diestrus (first column) and on GD12 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm. G. RNAscope images of endocervix from long-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In long-term tracing, tdTomato⁺ cells persist and give rise to secretory goblet cells that express Spdef and Muc5b in diestrus (second column) and in a second pregnancy at GD15 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm.

    Techniques Used: RNA Expression, Generated, Flow Cytometry, Derivative Assay, RNAscope, Staining



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    krt8 cre ert2 17blpn j mice  (Jackson Laboratory)
    86
    Jackson Laboratory krt8 cre ert2 17blpn j mice
    A. Schematic of lineage-tracing strategy using <t>Krt8-Cre</t> <t>ERT2</t> ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.
    Krt8 Cre Ert2 17blpn J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt8+cre+ert2+17blpn+j+mice/cre+ert2+mice+ubc/bio_rxiv__64898__2026__04__28__721179-199-1-8
    Average 86 stars, based on 1 article reviews
    krt8 cre ert2 17blpn j mice - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Schematic of lineage-tracing strategy using Krt8-Cre ERT2 ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.

    Journal: bioRxiv

    Article Title: Olfactomedin4 marks luminal progenitor cells that give rise to secretory cell lineage in the mouse cervix

    doi: 10.64898/2026.04.28.721179

    Figure Lengend Snippet: A. Schematic of lineage-tracing strategy using Krt8-Cre ERT2 ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.

    Article Snippet: The Krt8-Cre/ ERT2 (17Blpn/J) mice were sourced from Jackson Laboratories.

    Techniques: RNA Expression, Staining, Marker

    A. Schematic of lineage tracing strategy using Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. B. RNA expression of tdTomato+ (red), Spdef (cyan) and Muc5b (green) in endocervical tissue from nonpregnant (diestrus) and pregnancy (GD 6,12,15, 18 and IL). All three markers were detected in the same section, with each signal displayed in separate panels. Images are representative of n≥ 3 biological replicates per time point and scale bars =100 µm. C. Schematic of workflow used to generate organoid cultures from tdTomato negative and tdTomato positive epithelial populations from the cervix of nonpregnant diestrus Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. D. Organoids generated from flow cytometry sorted tdTomato+ and tdTomato- epithelial populations. tdTomato+ derived organoids retained tdTomato signal, whereas tdTomato-progenitors formed organoids that did not express tdTomato. n = 3 biological replicates and scale bars =100 µm. E. Schematic of short-term and long-term lineage-tracing strategy using Olfm4 Cre ERT2 ; Rosa26 tdTomato mice (8-week-old females). Short-term tracing (top): Tamoxifen (Tmx) was administered at estrus, diestrus, or GD11, and tissues were collected 24-48 hrs later at the subsequent time points (estrus to diestrus, diestrus to estrus, GD11 to GD12). Long-term tracing (bottom): Tmx was administered at the NP stage, followed by a first pregnancy. After the first pregnancy, tissues were collected at two stages of the estrous cycle, or on GD15 of a second pregnancy. F. RNAscope images of endocervix from short-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In short-term tracing, tdTomato⁺ cells are present and co-localize with goblet markers during diestrus (first column) and on GD12 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm. G. RNAscope images of endocervix from long-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In long-term tracing, tdTomato⁺ cells persist and give rise to secretory goblet cells that express Spdef and Muc5b in diestrus (second column) and in a second pregnancy at GD15 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm.

    Journal: bioRxiv

    Article Title: Olfactomedin4 marks luminal progenitor cells that give rise to secretory cell lineage in the mouse cervix

    doi: 10.64898/2026.04.28.721179

    Figure Lengend Snippet: A. Schematic of lineage tracing strategy using Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. B. RNA expression of tdTomato+ (red), Spdef (cyan) and Muc5b (green) in endocervical tissue from nonpregnant (diestrus) and pregnancy (GD 6,12,15, 18 and IL). All three markers were detected in the same section, with each signal displayed in separate panels. Images are representative of n≥ 3 biological replicates per time point and scale bars =100 µm. C. Schematic of workflow used to generate organoid cultures from tdTomato negative and tdTomato positive epithelial populations from the cervix of nonpregnant diestrus Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. D. Organoids generated from flow cytometry sorted tdTomato+ and tdTomato- epithelial populations. tdTomato+ derived organoids retained tdTomato signal, whereas tdTomato-progenitors formed organoids that did not express tdTomato. n = 3 biological replicates and scale bars =100 µm. E. Schematic of short-term and long-term lineage-tracing strategy using Olfm4 Cre ERT2 ; Rosa26 tdTomato mice (8-week-old females). Short-term tracing (top): Tamoxifen (Tmx) was administered at estrus, diestrus, or GD11, and tissues were collected 24-48 hrs later at the subsequent time points (estrus to diestrus, diestrus to estrus, GD11 to GD12). Long-term tracing (bottom): Tmx was administered at the NP stage, followed by a first pregnancy. After the first pregnancy, tissues were collected at two stages of the estrous cycle, or on GD15 of a second pregnancy. F. RNAscope images of endocervix from short-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In short-term tracing, tdTomato⁺ cells are present and co-localize with goblet markers during diestrus (first column) and on GD12 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm. G. RNAscope images of endocervix from long-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In long-term tracing, tdTomato⁺ cells persist and give rise to secretory goblet cells that express Spdef and Muc5b in diestrus (second column) and in a second pregnancy at GD15 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm.

    Article Snippet: The Krt8-Cre/ ERT2 (17Blpn/J) mice were sourced from Jackson Laboratories.

    Techniques: RNA Expression, Generated, Flow Cytometry, Derivative Assay, RNAscope, Staining